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human testis large insert cdna library  (TaKaRa)


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    Structured Review

    TaKaRa human testis large insert cdna library
    Human Testis Large Insert Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+testis+large+insert+cdna+library/Human+Testis+QUICK-Clone+cDNA/pmc03730229__emboj2013148s1-1-48-54
    Average 93 stars, based on 434 article reviews
    human testis large insert cdna library - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    cDNA Library Assay:

    Article Title: A Gene Encoding Sialic-Acid-Specific 9-O-Acetylesterase Found in Human Adult Testis
    Article Snippet: .. Human Testis Large-Insert cDNA Library (Clontech, Calif) was first converted into plasmid cDNA Library, and then an arrayed cDNA library in 96-well plates was made according to the method of Munroe [ , ]. ..

    Article Title: Identification of a novel lipase gene mutated in lpd mice with hypertriglyceridemia and associated with dyslipidemia in humans.
    Article Snippet: .. Identification of human LPDL and murine lpdlr (lpdl related lipase) gene Using mouse lpdl gene fragments as probes to screen a human testis large insert cDNA library (Clontech), four positive clones were identified. ..

    Article Title: A Gene Encoding Sialic-Acid-Specific 9-O-Acetylesterase Found in Human Adult Testis
    Article Snippet: This DNA was reamplified with the same combination of primers and then subcloned into Pinpoint Xa1-T vector (Promega, USA). cDNA library screening The colonies of full-length cDNA were screened by PCR. .. Human Testis Large-Insert cDNA Library (Clontech, Calif) was first converted into plasmid cDNA Library, and then an arrayed cDNA library in 96-well plates was made according to the method of Munroe [13, 14]. ..

    Article Title: Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes.
    Article Snippet: .. The plasmid containing sNORPEG from the human testis large insert cDNA library (HL5503U, Clontech) was used as a positive control of PCR amplification. ..

    Article Title: DNA polymerase κ-dependent DNA synthesis at stalled replication forks is important for CHK1 activation
    Article Snippet: .. The embryonic fibroblasts (MEF) used in this study were generated from homozygous mutant Pol -deficient mice described in (Schenten et al, 2002) Preparation of polyclonal anti-rabbit IgG antibody of human Pol The human POL K gene (GenBank accession number XM_003930.2), was amplified by polymerase chain reaction (PCR) from human testis large insert cDNA library (CLONTECH). .. The amplified fragments were digested with NcoI and BamHI and the resulting fragment was ligated into similarly digested vector pYG8582, which is the same as pET-16b (Novagen) but has the translational DB enhancer.

    Plasmid Preparation:

    Article Title: A Gene Encoding Sialic-Acid-Specific 9-O-Acetylesterase Found in Human Adult Testis
    Article Snippet: .. Human Testis Large-Insert cDNA Library (Clontech, Calif) was first converted into plasmid cDNA Library, and then an arrayed cDNA library in 96-well plates was made according to the method of Munroe [ , ]. ..

    Article Title: A Gene Encoding Sialic-Acid-Specific 9-O-Acetylesterase Found in Human Adult Testis
    Article Snippet: This DNA was reamplified with the same combination of primers and then subcloned into Pinpoint Xa1-T vector (Promega, USA). cDNA library screening The colonies of full-length cDNA were screened by PCR. .. Human Testis Large-Insert cDNA Library (Clontech, Calif) was first converted into plasmid cDNA Library, and then an arrayed cDNA library in 96-well plates was made according to the method of Munroe [13, 14]. ..

    Article Title: Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes.
    Article Snippet: .. The plasmid containing sNORPEG from the human testis large insert cDNA library (HL5503U, Clontech) was used as a positive control of PCR amplification. ..

    Clone Assay:

    Article Title: Identification of a novel lipase gene mutated in lpd mice with hypertriglyceridemia and associated with dyslipidemia in humans.
    Article Snippet: .. Identification of human LPDL and murine lpdlr (lpdl related lipase) gene Using mouse lpdl gene fragments as probes to screen a human testis large insert cDNA library (Clontech), four positive clones were identified. ..

    Positive Control:

    Article Title: Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes.
    Article Snippet: .. The plasmid containing sNORPEG from the human testis large insert cDNA library (HL5503U, Clontech) was used as a positive control of PCR amplification. ..

    Polymerase Chain Reaction:

    Article Title: Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes.
    Article Snippet: .. The plasmid containing sNORPEG from the human testis large insert cDNA library (HL5503U, Clontech) was used as a positive control of PCR amplification. ..

    Article Title: DNA polymerase κ-dependent DNA synthesis at stalled replication forks is important for CHK1 activation
    Article Snippet: .. The embryonic fibroblasts (MEF) used in this study were generated from homozygous mutant Pol -deficient mice described in (Schenten et al, 2002) Preparation of polyclonal anti-rabbit IgG antibody of human Pol The human POL K gene (GenBank accession number XM_003930.2), was amplified by polymerase chain reaction (PCR) from human testis large insert cDNA library (CLONTECH). .. The amplified fragments were digested with NcoI and BamHI and the resulting fragment was ligated into similarly digested vector pYG8582, which is the same as pET-16b (Novagen) but has the translational DB enhancer.

    Amplification:

    Article Title: Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes.
    Article Snippet: .. The plasmid containing sNORPEG from the human testis large insert cDNA library (HL5503U, Clontech) was used as a positive control of PCR amplification. ..

    Article Title: DNA polymerase κ-dependent DNA synthesis at stalled replication forks is important for CHK1 activation
    Article Snippet: .. The embryonic fibroblasts (MEF) used in this study were generated from homozygous mutant Pol -deficient mice described in (Schenten et al, 2002) Preparation of polyclonal anti-rabbit IgG antibody of human Pol The human POL K gene (GenBank accession number XM_003930.2), was amplified by polymerase chain reaction (PCR) from human testis large insert cDNA library (CLONTECH). .. The amplified fragments were digested with NcoI and BamHI and the resulting fragment was ligated into similarly digested vector pYG8582, which is the same as pET-16b (Novagen) but has the translational DB enhancer.

    Generated:

    Article Title: DNA polymerase κ-dependent DNA synthesis at stalled replication forks is important for CHK1 activation
    Article Snippet: .. The embryonic fibroblasts (MEF) used in this study were generated from homozygous mutant Pol -deficient mice described in (Schenten et al, 2002) Preparation of polyclonal anti-rabbit IgG antibody of human Pol The human POL K gene (GenBank accession number XM_003930.2), was amplified by polymerase chain reaction (PCR) from human testis large insert cDNA library (CLONTECH). .. The amplified fragments were digested with NcoI and BamHI and the resulting fragment was ligated into similarly digested vector pYG8582, which is the same as pET-16b (Novagen) but has the translational DB enhancer.

    Mutagenesis:

    Article Title: DNA polymerase κ-dependent DNA synthesis at stalled replication forks is important for CHK1 activation
    Article Snippet: .. The embryonic fibroblasts (MEF) used in this study were generated from homozygous mutant Pol -deficient mice described in (Schenten et al, 2002) Preparation of polyclonal anti-rabbit IgG antibody of human Pol The human POL K gene (GenBank accession number XM_003930.2), was amplified by polymerase chain reaction (PCR) from human testis large insert cDNA library (CLONTECH). .. The amplified fragments were digested with NcoI and BamHI and the resulting fragment was ligated into similarly digested vector pYG8582, which is the same as pET-16b (Novagen) but has the translational DB enhancer.



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